This way, you can design primers to be used when simulating various procedures. Last but not least, SnapGene includes thermodynamic algorithms that can be employed to determine duplex alignments and melting temperatures. The latter function is extremely useful if you need to identify viable enzyme sets. All your steps while performing a particular procedure are automatically documented, so you will have a clear log of all your steps.įurthermore, SnapGene can be used to generate agarose gel simulations, or to highlight the sites that are tampered with by methylation. SnapGene integrates support for in-fusion cloning, Gibson assembly procedures, restriction cloning, and PCR and Mutagenesis. Process the data by employing specific methods The application can highlight the potential Open Reading Frames on its own, but also allows you to adjust the parameters for more accurate results. Moreover, SnapGene integrates various customization options and allows you to view specific segments, to change the directionality or the color tags. The application is following the GenBank standards when it comes to generating the representations. Within the SnapGene user interface, you get to visualize the DNA sequence structure. View, document and annotate DNA sequences Moreover, you can also use the free SnapGene Viewer utility to share the results with your colleagues. The data can then by exported to file formats compatible with other popular software solutions designed to work with DNA sequences. ![]() Sticky ends from different SapI sites may not be compatible.SnapGene is a versatile molecular biology software that enables you to documents DNA constructs without having to deal with complicated tools or workflows. Sticky ends from different BspQI sites may not be compatible. Sticky ends from different BstAPI sites may not be compatible.Įfficient cleavage requires at least two copies of the NarI recognition sequence.Įfficient cleavage requires at least two copies of the PluTI recognition sequence. ![]() G C A N N N N N T G C C G T N N N N N A C G Prolonged incubation with NdeI may lead to removal of additional nucleotides. ![]() Sticky ends from different PfoI sites may not be compatible. Sticky ends from different AlwNI sites may not be compatible. Sticky ends from different AhdI sites may not be compatible.
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